Antimicrobial Susceptibility Pattern of Biofilm forming Pseudomonas aeruginosa Isolated from Noncritical Surfaces in a Tertiary Healthcare Facility in South Eastern Nigeria

Agbo Ejiofor C.¹, Ejinaka Obiora. R.², Obeta M. Uchejeso^3, , Lote-Nwaru Ifeoma E.⁴, Ibanga Imoh E³ and Dajok D. Godfrey⁴

¹Department of Medical Laboratory Services, Federal Medical Centre, Umuahia-Nigeria

²Department Parasitology, Federal School of Medical Laboratory Science, Jos-Nigeria

³Department of Chemical Pathology, Federal School of Medical Laboratory Science, Jos-Nigeria

⁴Department of Medical Microbiology, Federal School of Medical Laboratory Science, Jos-Nigeria

Cite this paper

Agbo Ejiofor C., Ejinaka Obiora. R., Obeta M. Uchejeso, Lote-Nwaru Ifeoma E., Ibanga Imoh E and Dajok D. Godfrey. Antimicrobial Susceptibility Pattern of Biofilm forming Pseudomonas aeruginosa Isolated from Noncritical Surfaces in a Tertiary Healthcare Facility in South Eastern Nigeria. American Journal of Public Health Research. 2020; 8(3):87-92. doi: 10.12691/AJPHR-8-3-2

Abstract

Background: The presence of biofilm forming Pseudomonas species on noncritical surfaces in various hospital areas are the basis of Healthcare Associated Infections. Justification: The Healthcare associated infections are on the increase, affecting both care givers and patients with many showing resistant to many antibiotics and therefore calls for study for better understanding of the susceptibility of Pseudomonas aeruginosa isolated from noncritical surfaces in the facility. Aim and objectives: The study was to assess the susceptibility of commonly prescribed antibiotics in the south eastern healthcare facility and to be able to educate the staff, students and patients. Methodology: The study used an experimental design carried out in 800 beds capacity Federal Medical Center, Umuahia, and South East Nigeria. These bacteria were isolated using the swab to collect samples for analysis. Samples were collected from different noncritical surfaces surrounding hospitalized patients and equipment in the tertiary healthcare facility. The 450 positive samples out of the 1314 samples collected were analyzed for bacterial isolation and identification using bacterial cultural and microscopic identification techniques, biochemical tests and the Microbact 24E assay. Result: Biofilm forming Pseudomonas aeruginosa were identified through crystal violet assay while Antimicrobial susceptibility test was done using agar well diffusion method which was carried out on the isolated biofilm forming Pseudomonas aeruginosa. Conclusion: The susceptibility showed that biofilm forming Pseudomonas aeruginosa isolates were resistant to Gentamicin and Augmentin, but sensitive to Vancomycin, Azithromycin and Meropenem. Pseudomonas aeruginosa has the highest potential to form biofilms and could be recognized as a major agent of nosocomial infections in healthcare facilities in South East. Its notable resistance to some major antibiotics used in those centres calls for an urgent need for caregivers to carry out susceptibility testing before antibiotic prescription.

Keywords

antimicrobial susceptibility, biofilm forming pseudomonas aeruginosa, hospital, South East-Nigeria

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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